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fluorescent microscopy nikon dapi-fitc-tritc filter combinations  (Nikon)


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    Nikon fluorescent microscopy nikon dapi-fitc-tritc filter combinations
    Fluorescent Microscopy Nikon Dapi Fitc Tritc Filter Combinations, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescence+microscopy+with+dapi/pm38451229-134-7-9
    Average 90 stars, based on 1 article reviews
    fluorescent microscopy nikon dapi-fitc-tritc filter combinations - by Bioz Stars, 2026-11
    90/100 stars

    Images

    Related Articles

    other:

    Article Title: In vitro and in silico evaluation of the serrapeptase effect on biofilm and amyloids of Pseudomonas aeruginosa.
    Article Snippet: Staining was performed for 15 min in the dark, and then biofilms were washed thoroughly with PBS and observed under a Nikon Eclipse Ci fluorescence microscope equipped with a Color Camera Nikon DS-Fi3 (× 20, FITC filter, 80 ms exposure for ThT/ × 20, DAPI filter, 30 ms exposure for DAPI).

    Article Title: Fluorescent carbon nanodot-based rapid cell nucleus staining and application for diagnosis
    Article Snippet: Finally, the fluorescence imaging was performed under a Nikon NI–U microscope with a FITC/DAPI filter and 100-w mercury lamp.

    Article Title: Cortisol regulates neonatal lung development via Smoothened.
    Article Snippet: Nuclei was labeled with DAPI and finally photographed by Nikon A1 confocal fluorescence microscopy.

    Article Title: In vitro and in silico evaluation of the serrapeptase effect on biofilm and amyloids of Pseudomonas aeruginosa
    Article Snippet: Staining was performed for 15 min in the dark, and then biofilms were washed thoroughly with PBS and observed under a Nikon Eclipse Ci fluorescence microscope equipped with a Color Camera Nikon DS-Fi3 (× 20, FITC filter, 80 ms exposure for ThT/ × 20, DAPI filter, 30 ms exposure for DAPI).

    Fluorescence:

    Article Title: Influenza A Virus and Mycoplasma pneumoniae Coinfection Mediates Immune Dysregulation and Exacerbates Disease Severity
    Article Snippet: Subsequently, the process was repeated for rabbit anti-CD8 (1:1000; Servicebio, Wuhan, China) using the same secondary antibody and IF-488 conjugated tyramide (TSA 488,Servicebio, Wuhan, China). .. Nuclei were counterstained with DAPI, and images were captured using a Nikon Eclipse C1 upright fluorescence microscope and analyzed with Aipathwell v2 software. ..

    Article Title: Role of UVA-driven cellular senescence via mTOR activation in dihydrotestosterone-induced hair loss in androgenetic alopecia mouse model.
    Article Snippet: Background: Androgenetic alopecia (AGA) is one of the most common forms of hair loss, and recent studies suggest that dihydrotestosterone (DHT)-induced senescence of dermal papilla cells (DPCs) plays a crucial role in its pathogenesis.. Clinically, we previously observed an overlap between areas exposed to ultraviolet (UV) radiation and regions affected by androgenetic hair loss.. However, the relationship between UVA radiation and AGA onset remains unclear.

    Article Title: Influenza A Virus and Mycoplasma pneumoniae Coinfection Mediates Immune Dysregulation and Exacerbates Disease Severity
    Article Snippet: Subsequently, the process was repeated for rabbit anti-CD8 (1:1000; Servicebio, Wuhan, China) using the same secondary antibody and IF-488 conjugated tyramide (TSA 488,Servicebio, Wuhan, China). .. Nuclei were counterstained with DAPI, and images were captured using a Nikon Eclipse C1 upright fluorescence microscope and analyzed with Aipathwell v2 software. ..

    Article Title: Niraparib plays synergistic antitumor effects with NRT in a mouse ovarian cancer model with HRP.
    Article Snippet: Objective: PARPi offers less clinical benefit for HRP patients compared to HRD patients.. PARPi has an immunomodulatory function.. NRT therapy targets tumor neoantigens without off-target immune toxicity.

    Microscopy:

    Article Title: Influenza A Virus and Mycoplasma pneumoniae Coinfection Mediates Immune Dysregulation and Exacerbates Disease Severity
    Article Snippet: Subsequently, the process was repeated for rabbit anti-CD8 (1:1000; Servicebio, Wuhan, China) using the same secondary antibody and IF-488 conjugated tyramide (TSA 488,Servicebio, Wuhan, China). .. Nuclei were counterstained with DAPI, and images were captured using a Nikon Eclipse C1 upright fluorescence microscope and analyzed with Aipathwell v2 software. ..

    Article Title: Role of UVA-driven cellular senescence via mTOR activation in dihydrotestosterone-induced hair loss in androgenetic alopecia mouse model.
    Article Snippet: Background: Androgenetic alopecia (AGA) is one of the most common forms of hair loss, and recent studies suggest that dihydrotestosterone (DHT)-induced senescence of dermal papilla cells (DPCs) plays a crucial role in its pathogenesis.. Clinically, we previously observed an overlap between areas exposed to ultraviolet (UV) radiation and regions affected by androgenetic hair loss.. However, the relationship between UVA radiation and AGA onset remains unclear.

    Article Title: Influenza A Virus and Mycoplasma pneumoniae Coinfection Mediates Immune Dysregulation and Exacerbates Disease Severity
    Article Snippet: Subsequently, the process was repeated for rabbit anti-CD8 (1:1000; Servicebio, Wuhan, China) using the same secondary antibody and IF-488 conjugated tyramide (TSA 488,Servicebio, Wuhan, China). .. Nuclei were counterstained with DAPI, and images were captured using a Nikon Eclipse C1 upright fluorescence microscope and analyzed with Aipathwell v2 software. ..

    Article Title: Niraparib plays synergistic antitumor effects with NRT in a mouse ovarian cancer model with HRP.
    Article Snippet: Objective: PARPi offers less clinical benefit for HRP patients compared to HRD patients.. PARPi has an immunomodulatory function.. NRT therapy targets tumor neoantigens without off-target immune toxicity.

    Software:

    Article Title: Influenza A Virus and Mycoplasma pneumoniae Coinfection Mediates Immune Dysregulation and Exacerbates Disease Severity
    Article Snippet: Subsequently, the process was repeated for rabbit anti-CD8 (1:1000; Servicebio, Wuhan, China) using the same secondary antibody and IF-488 conjugated tyramide (TSA 488,Servicebio, Wuhan, China). .. Nuclei were counterstained with DAPI, and images were captured using a Nikon Eclipse C1 upright fluorescence microscope and analyzed with Aipathwell v2 software. ..

    Article Title: Influenza A Virus and Mycoplasma pneumoniae Coinfection Mediates Immune Dysregulation and Exacerbates Disease Severity
    Article Snippet: Subsequently, the process was repeated for rabbit anti-CD8 (1:1000; Servicebio, Wuhan, China) using the same secondary antibody and IF-488 conjugated tyramide (TSA 488,Servicebio, Wuhan, China). .. Nuclei were counterstained with DAPI, and images were captured using a Nikon Eclipse C1 upright fluorescence microscope and analyzed with Aipathwell v2 software. ..

    Staining:

    Article Title: Role of UVA-driven cellular senescence via mTOR activation in dihydrotestosterone-induced hair loss in androgenetic alopecia mouse model.
    Article Snippet: Background: Androgenetic alopecia (AGA) is one of the most common forms of hair loss, and recent studies suggest that dihydrotestosterone (DHT)-induced senescence of dermal papilla cells (DPCs) plays a crucial role in its pathogenesis.. Clinically, we previously observed an overlap between areas exposed to ultraviolet (UV) radiation and regions affected by androgenetic hair loss.. However, the relationship between UVA radiation and AGA onset remains unclear.

    Imaging:

    Article Title: Role of UVA-driven cellular senescence via mTOR activation in dihydrotestosterone-induced hair loss in androgenetic alopecia mouse model.
    Article Snippet: Background: Androgenetic alopecia (AGA) is one of the most common forms of hair loss, and recent studies suggest that dihydrotestosterone (DHT)-induced senescence of dermal papilla cells (DPCs) plays a crucial role in its pathogenesis.. Clinically, we previously observed an overlap between areas exposed to ultraviolet (UV) radiation and regions affected by androgenetic hair loss.. However, the relationship between UVA radiation and AGA onset remains unclear.

    Immunofluorescence:

    Article Title: Niraparib plays synergistic antitumor effects with NRT in a mouse ovarian cancer model with HRP.
    Article Snippet: Objective: PARPi offers less clinical benefit for HRP patients compared to HRD patients.. PARPi has an immunomodulatory function.. NRT therapy targets tumor neoantigens without off-target immune toxicity.

    Labeling:

    Article Title: Niraparib plays synergistic antitumor effects with NRT in a mouse ovarian cancer model with HRP.
    Article Snippet: Objective: PARPi offers less clinical benefit for HRP patients compared to HRD patients.. PARPi has an immunomodulatory function.. NRT therapy targets tumor neoantigens without off-target immune toxicity.



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    Matisse Pharmaceuticals fluorescent microscopy images based on dapi nuclear staining
    Combining fluorescence microscopy with multiplex IMC data of colorectal tissue advances quality of single cell segmentation. a Cartoon describing MATISSE, a novel pipeline adding microscopic imaging to multiplex IMC analysis and downstream segmentation. In short: tissue sections on slides were stained using isotope-conjugated primary antibodies, DNA intercalator, and <t>DAPI.</t> The tissue was first scanned using <t>a</t> <t>fluorescent</t> microscope and then processed with IMC. Data produced by both techniques is aligned using the nuclear staining. Nuclear and membranous pixel probability maps are produced based on the fluorescent images and IMC data respectively. These probability maps are used to generate a segmentation map, where all detected cells are included. b Representative images of DNA intercalator on a colorectal tissue section analyzed by Ir193 labeling and IMC (left) or DAPI labeling and fluorescent microscopy (IF, right). c IMC-only (IMC) and MATISSE cell segmentation (MATISSE) were performed, and shown are the different predicted outlines on a representative image of Ir193 labeling. Arrows indicate areas with cell fragmentation. d Display of a large region of interest (ROI) showing an overlay of the predicted cell outlines (pink) upon IMC or MATISSE segmentation on a representative IMC image of DNA-Ir193 labeling of colorectal tissue. Highlighted in yellow is the approximate position of the basement membrane surrounding the epithelial monolayer. Scale bar 25 μm. e Cell density was calculated as the number of cells within a radius of 10 μM from the center of each single cell [ , ]. This number is displayed with a color code for each cell in the representative image
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    Image Search Results


    Combining fluorescence microscopy with multiplex IMC data of colorectal tissue advances quality of single cell segmentation. a Cartoon describing MATISSE, a novel pipeline adding microscopic imaging to multiplex IMC analysis and downstream segmentation. In short: tissue sections on slides were stained using isotope-conjugated primary antibodies, DNA intercalator, and DAPI. The tissue was first scanned using a fluorescent microscope and then processed with IMC. Data produced by both techniques is aligned using the nuclear staining. Nuclear and membranous pixel probability maps are produced based on the fluorescent images and IMC data respectively. These probability maps are used to generate a segmentation map, where all detected cells are included. b Representative images of DNA intercalator on a colorectal tissue section analyzed by Ir193 labeling and IMC (left) or DAPI labeling and fluorescent microscopy (IF, right). c IMC-only (IMC) and MATISSE cell segmentation (MATISSE) were performed, and shown are the different predicted outlines on a representative image of Ir193 labeling. Arrows indicate areas with cell fragmentation. d Display of a large region of interest (ROI) showing an overlay of the predicted cell outlines (pink) upon IMC or MATISSE segmentation on a representative IMC image of DNA-Ir193 labeling of colorectal tissue. Highlighted in yellow is the approximate position of the basement membrane surrounding the epithelial monolayer. Scale bar 25 μm. e Cell density was calculated as the number of cells within a radius of 10 μM from the center of each single cell [ , ]. This number is displayed with a color code for each cell in the representative image

    Journal: BMC Biology

    Article Title: MATISSE: a method for improved single cell segmentation in imaging mass cytometry

    doi: 10.1186/s12915-021-01043-y

    Figure Lengend Snippet: Combining fluorescence microscopy with multiplex IMC data of colorectal tissue advances quality of single cell segmentation. a Cartoon describing MATISSE, a novel pipeline adding microscopic imaging to multiplex IMC analysis and downstream segmentation. In short: tissue sections on slides were stained using isotope-conjugated primary antibodies, DNA intercalator, and DAPI. The tissue was first scanned using a fluorescent microscope and then processed with IMC. Data produced by both techniques is aligned using the nuclear staining. Nuclear and membranous pixel probability maps are produced based on the fluorescent images and IMC data respectively. These probability maps are used to generate a segmentation map, where all detected cells are included. b Representative images of DNA intercalator on a colorectal tissue section analyzed by Ir193 labeling and IMC (left) or DAPI labeling and fluorescent microscopy (IF, right). c IMC-only (IMC) and MATISSE cell segmentation (MATISSE) were performed, and shown are the different predicted outlines on a representative image of Ir193 labeling. Arrows indicate areas with cell fragmentation. d Display of a large region of interest (ROI) showing an overlay of the predicted cell outlines (pink) upon IMC or MATISSE segmentation on a representative IMC image of DNA-Ir193 labeling of colorectal tissue. Highlighted in yellow is the approximate position of the basement membrane surrounding the epithelial monolayer. Scale bar 25 μm. e Cell density was calculated as the number of cells within a radius of 10 μM from the center of each single cell [ , ]. This number is displayed with a color code for each cell in the representative image

    Article Snippet: We observed that incorporating fluorescent microscopy images based on DAPI nuclear staining into MATISSE workflow resulted in superior visual and signal intensity-based separation of nuclei in dense areas (Fig. b, Additional file : Fig S1B).

    Techniques: Fluorescence, Microscopy, Multiplex Assay, Imaging, Staining, Produced, Labeling, Membrane